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Allergy & Food Intolerance
Diagnostics
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Food Intolerance Diagnostic ELISA Kits for the detection of specific IgG4 and specific IgG
![null](https://www.dst-diagnostic.com/wp-content/uploads/2016/06/microtiter_plate3.png)
Test principle
Allergen extracts from different foods are bound to the surface of the microtiter plate wells. For testing, a diluted sample (serum or plasma) is added to the wells. Specific IgG4 antibodies of the sample bind to the antigens in the wells during incubation. Non-bound components of serum or plasma are washed away after incubation of the sample. Horseradish peroxidase- coupled anti-IgG4 antibodies (conjugate) are added and bind to the IgG4 antibodies of the sample, the standards or controls. Subsequently, unbound detection antibodies are washed away. The color substrate TMB (3,3‘,5,5‘-tetramethylbenzidine) is added, which is converted by the horseradish peroxidase conjugate. The reaction is stopped by adding a stop solution. A yellow dye is formed and the respective intensity correlates with the proportional amount of locally-bound antibody.
The identified units/mL can be assigned to the respective intensity and provide the level of specific IgG4 sensitization (See table below).
sIgG4/sIgG class | sIgG4/sIgG [Unit/ml] |
1 | 0,35 - 0,70 |
2 | 0,70 - 3,50 |
3 | 3,50 - 17,50 |
4 | 17,50 - 50,00 |
5 | 50,00 - 100,00 |
6 | > 100,00 |