Allergy & Food Intolerance

ALLERG4Y LINE Kit for in-vitro diagnosis of food intolerances

Food intolerances are a growing burden worldwide. To address this, DST GmbH has designed nitro-cellulose based line strips for detection of specific IgG4. The assay is reliable, fast and cost effective. Multiple food panels and -sizes are available.

Features and Benefits

  • Requires only 75 microlitres of serum or plasma per strip and patient
  • Total assay time of approx. 90 minutes
  • Detection range of specific IgG4 starting from 0.35 kUA/L
  • No cross reactions with other immunoglobulins
  • Inter-assay precision: mean coefficient of variation <10%
  • Comes with a quadrilingual step-by-step quick guide for carrying out the assay and evaluation sheet (German, English, Spanish and Portuguese)


Test principle

Food extracts of the dedicated panel are embedded in nitro-cellulose strip membranes. For testing, a diluted sample (serum or plasma) is added to the incubation tray. Specific IgG4 antibodies of the sample bind to the spotted antigens on the membrane during incubation, and non-bound components of serum or plasma are washed away after incubation. Subsequently, anti-human IgG4 antibodies coupled horseradish peroxidase is added and bind to the IgG4 antibodies of the sample and the standards. Then the unbound detection antibodies are washed away. The color substrate TMB (3,3‘,5,5‘-tetramethylbenzidine) is added, which is converted by the horseradish peroxidase conjugate. The reaction is stopped by adding a stop solution. A dark colored dye is formed and the respective intensity correlates with the proportional amount of locally-bound antibody.The identified kUA/L can be assigned to the respective intensity and provide the level of specific IgG4 sensitization (See table below).

The identified untis/ml of IgG4 represent the level of food sensitisation

sIgG4-Class (Range)sIgG4-Unit [Units/mL]
10,35 - 0,70
20,70 - 3,50
33,50 - 17,50
417,50 - 50,00
550,00 - 100,00
6≥ 100
Reference: Package leaflet